|
KMID : 0364920140390030134
|
|
Journal of Radiation Protection and Research
2014 Volume.39 No. 3 p.134 ~ p.141
|
|
|
Effects of Low-Dose Fractionated Total Body Irradiation on Murine Immune System
|
|
Kim Mi-Hyoung
Ryu Sang-Young
Lim Dae-Seog
Song Jie-Young
|
|
|
Abstract
|
|
|
|
Along with the wide use of radiotherapy in cancer treatment, there is growing interest in beneficial effect of low-dose irradiation (LDI) in cancer therapy. Therefore, we investigate how LDI affects immune responses in mice model. Total body irradiation (TBI) on C57BL/6 mice was given at low-dose rate of 1 mGy?min-1 using 137Cs source at three times for consecutive three days. Hematological examination, total cell numbers of spleen, populations and characteristics of splenocytes were determined. Total numbers of RBC or platelet in irradiated mice showed no significant changes. WBC counts were decreased in a dose-dependent manner 2 days after TBI, however, these differences are gradually waned until 28 days. Dose-dependent decrease in the number of splenocytes of TBI mice at day 2 was also improved as time progressed. While the level of Foxp3 mRNA was decreased, the frequency of CD4+ T cells and CD69+ cells in spleen was increased at day 2 and 14. Fractionated low-dose TBI on mice exhibited normal body weight with no distinguishable behavior during whole experimental periods. These results suggest that some parameters of immune system could be altered and evaluated by fractionated low-dose TBI and be used to broaden boundary of low dose radiation research.
|
|
|
KEYWORD
|
|
|
Low-dose irradiation, Total body irradiation, Immune modulation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|